Enantiomer Identification (HPLC/SFC)
BOC Sciences is a professional service provider of chiral compound analysis and identification at home and abroad. We not only have professional technical level, but also good after-sales service and high-quality solutions.
Chirality plays an extremely crucial role in life sciences because many major biological activities are achieved through strict chiral matching to generate molecular recognition. Therefore, it is undoubtedly of great significance to study the chiral molecular system and mechanism of action and continuously develop new technologies and new methods for chiral research.The analysis and identification of chiral compounds are one of the hot issues in the current analytical chemistry community. Modern chromatographic separation technology shows great superiority in the separation and determination of enantiomers.
HPLC is the most widely used method for the separation and determination of enantiomers. It is especially suitable for the analysis of chiral materials with strong polarity and poor thermal stability. Supercritical fluid chromatography (SFC) is a new chromatographic technique. Because it has the advantages that gas and liquid phases do not have, it can separate and analyze some objects that gas and liquid chromatography cannot solve.
Fig.1 Analysis of a compound in HPLC
HPLC uses a high-pressure infusion system to pump single or mixed solvents, buffers and other liquid mobile phases with different polarities into a chromatographic column equipped with a stationary phase. The components are separated in the chromatographic column, and then enter the detector for detection, so as to realize the analysis and identification of the sample. The identification and quantification of compounds in HPLC are determined by chromatograms. Usually, the x-axis represents retention time, and the y-axis depends on the detection method. An ultraviolet detector is used to measure the intensity of absorbance. In addition, other types of detectors, such as mass spectrometry (MS), nuclear magnetic resonance (NMR), and fluorescence detectors, can also be used.
The method for identifying compounds by HPLC and SFC is basically the same. It is to determine what each chromatogram peak represents, and thus determine the composition of the sample mixture made up of these components. The retention value is related to the structure and properties of the component and can be used for compound identification. With pure substances as a control, each substance has its own retention value (including retention time, retention volume, adjustment of retention time, adjustment of retention volume, etc.) under certain chromatographic conditions. First determine the retention time or retention distance of each chromatographic peak in the unknown, then inject a pure substance to be measured into the chromatograph, compare the retention values of the two. If the two retention values are the same, then the unknown can be determined. Pure substances are known to be the same substance. It is also possible to add a pure substance directly into the sample and then into the chromatograph. If the peak height of a component peak increases, it means that this chromatographic peak is the pure substance added. This method is also called the pure substance addition method. In general, HPLC and SFC supported columns can separate isomeric mixtures with multiple chiral atoms. But if the two enantiomers differ very little, ordinary operations are difficult to distinguish, then you need to use excellent results. The chiral column is specifically designed to deal with this situation.
Our Service
- Enantiomeric purity (ee%) determination
- Chiral analysis method development and validation
- Impurity inspection
- Specific component content analysis
- Structure Identification
BOC Sciences has advanced equipment and professional technicians who can provide you with analytical and appraisal services for specific chiral compounds. If you need services in this area, please contact us. We will wholeheartedly serve you.