Bioconjugation
BOC Sciences is an expert at various bioconjugation techniques and works closely with our customers. We can provide you with high quality standards and punctual delivery of bioconjugation services. Our professional chemistry team is well trained to focus on the bioconjugation techniques.
Figure.1 the conjugation of biomolecule
What is Bioconjugation?
The domain of bioconjugation techniques is dedicated to the precise and deliberate covalent attachment of two or more molecules, each of which is imbued with its own characteristic properties and function. The molecules are typically as disparate as can be - one may be a biomolecule like a protein, DNA or RNA while the other could very well be a non-biological molecule like a small molecule, peptide, or polymer. The crux of bioconjugation lies in the creation of a novel molecule, with an intricate blend of both of their unique qualities, resulting in a new substance with unparalleled functionality and application potential.
Types of Bioconjugation Techniques
The fascinating and multidimensional field of bioconjugation techniques encompasses a myriad of diverse strategies for the precise covalent attachment of two or more molecules, each with their own unique chemical properties and biological functions.
Among the plethora of techniques at hand, crosslinking is a particularly salient method whereby molecules are joined together through a chemical bond, such as a disulfide bond or an amide bond, by leveraging an array of crosslinking agents, including but not limited to, glutaraldehyde and carbodiimides.
Another compelling technique, click chemistry, involves the use of highly reactive and bioorthogonal chemical groups that can selectively and rapidly react with each other. The copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction, in particular, has gained widespread popularity as a prominent click chemistry reaction.
Carbonyl chemistry is yet another intriguing bioconjugation technique, which entails the reaction of carbonyl groups, such as aldehydes and ketones, with a nucleophilic group on another molecule, such as an amine or hydrazine, primarily for the conjugation of small molecules and proteins.
Thiol chemistry, on the other hand, revolves around the reaction of thiol groups on cysteine residues of a protein with a reactive group on another molecule, such as a maleimide or iodoacetamide, and is particularly useful for protein conjugation and modification.
Finally, the genetic engineering technique utilizes recombinant DNA technology to manipulate the sequence of a biomolecule, such as a protein, to introduce new reactive groups that can be utilized for bioconjugation.
Our Bioconjugation Services
Biomolecule labeling generally refers to cross-linking or modification in any form, whose purpose is to conjugate a chemical group with target molecules through single, bifunctional or heterofunctional cross linkers to aid in detection. The chemical group can be fluorescent, biotin, haptens, nanoparticles and other target functional group and the target molecules can be proteins, nucleic acids and other biomolecules. Biomolecule labeling plays an important role for the elucidation of complicated biological processes and is highly useful in the construction of various small-molecule probes to explore complex biological systems. Labeled biomolecules can be applied as signaling molecules, tools in molecular biology, drugs in medicine. BOC Sciences can offer you with various biomolecule labeling services such as biotin labeling, fluorescent labeling, and enzyme labeling. What’s more, we can even offer custom signal molecule conjugation service which can provide new small molecules, modification of existing molecules according to your demand.
Antibodies are highly specific molecules which can bind with high affinity to their cognate antigens. As tools, antibodies can be the primary choice as targeting moieties in targeted drug delivery and immunoassay or detection applications. Antibody conjugation refers to the conjugation of antibodies or their fragments with biologically active molecules such as nucleic acids, proteins or any molecular groups, which can improve or add valuable physico-chemical, toxicological and pharmacological properties. Antibody conjugates are important in many fields of medicine and biological research. For example, antibody-drug conjugates (ADCs) are becoming an important next generation class of therapeutics for cancer treatment and achieve tremendous clinical success for cancer therapy because of careful optimization of several parameters, including monoclonal antibody specificity (mAbs), drug potency, linker technology, and the stoichiometry and placement of conjugated drugs. In addition to antibody-drug conjugates, BOC Sciences also achieves other antibody conjugates with antibody conjugation techniques such as antibody-polymer conjugates, antibody-enzyme conjugates.
BOC Sciences also provides additional conjugation services in addition to what we mentioned above, such as peptide bioconjugation, protein bioconjugation, enzyme bioconjugation, liposome bioconjugation, PEGylation, and nano to macro particles. These conjugation services can be used in various biological applications. For example, the bioconjugation between enzyme such as Horseradish Peroxidase (HRP) and oligonucleotides, peptides or proteins acts as signal molecules in various applications.
Our Advantages
- Varieties of bioconjugation services
- Professional bioconjugation chemists
- Custom bioconjugation services
- Quickly and accurately