Biotinylation of lipids is the process of attaching biotin to lipids with covalent bonds. Since biotin is small, biotinylation is usually rapid and specific and does not easily interfere with the intrinsic function of the molecule. Biotin binds to streptavidin or anti-biotin proteins with very high affinity, rapidity and high specificity, therefore, biotinylated lipids are widely used in drug delivery and targeting, biosensing and detection, membrane protein research and cell imaging and tracking. Through specific interactions with biotin-binding proteins, biotinylated lipids can achieve specific target localization, detection and imaging in biological systems, providing important tools for research and applications in related fields.
Biotinylated lipids have a biotin portion built into one of the fatty acid chains of the lipid. One of the main roles of these lipids is the coupling of recombinant ligands to artificial lipid bilayers. Artificial lipid bilayers are used to mimic the interaction between platelets and membrane-bound ligands. Biotinylated lipids are used to couple monobiotinylated recombinant ligands to the upper leaflets of the artificial lipid bilayer.
The biotinylated lipids services offered by BOC Sciences can often be customized to meet a client's specific needs. This may include customized biotinylated lipid structures, yield requirements, purity requirements, and application-specific needs.
Biotinylation reagents can be selected and customized based on the customer's needs and the application of the project.
(1) Amine biotinylation
These reagents selectively and efficiently label biotin to the primary amino group on the surface of the target macromolecule. These reactive amines can be applied to materials such as polymers (e.g. PEI) and nanoparticles.
(2) Thiol biotinylation
These reagents contain functional groups that react with free sulfhydryl groups such as maleamide, haloacetyl or activated sulfhydryl groups.
(3) Carbonyl biotinylation
Carbonyl compounds, including aldehydes and ketones, react with hydrazides or hydroxylamines to form stable couplings in neutral aqueous solutions. Using this reaction principle, target molecules with carbonyl groups can be biotinylated.
(4) Carboxyl biotinylation
Polymers with free carboxylic acid groups, particles with carboxylic acid groups on the surface, etc. can be biotinylated. Stable biotinylation labeling can be achieved by reacting with this type of biotinylation reagent.
Binding of lipids to biotinylated reagents is carried out by chemical reactions, solid-phase meals, or enzyme-catalyzed reactions.
Biotinylated lipids are regulated by adjusting the structure and composition of the lipid. Biotinylated lipids retain other properties of lipids, such as self-assembly ability, surface activity, micelle formation, etc., which can extend their potential for applications in nanotechnology and biomaterials.
BOC Sciences performs purification of biotinylated lipids in a variety of ways and can be characterized by mass spectrometry, nuclear magnetic resonance, and high-performance liquid chromatography (HPLC) analysis.
Biotinylated lipids offer the advantages of specific binding, biocompatibility, controllability and multifunctionality, drug-carrying capacity, and tunable physicochemical properties. BOC Sciences can provide customers with professional biotinylated lipids products and optimize their product solutions, if you are interested in our services, please feel free to contact us.