Peptide Synthesis

Peptide Synthesis

Peptides are central compounds in the pharmaceutical industry, providing both final medications and lead compounds for the preparation of peptidomimetic or non-peptidic pharmaceuticals. Therapeutic peptides are recognised as being extremely specific in their binding to in vivo targets, resulting in them often being highly potent and very selective, whilst having very few negative side effects. Currently, there are more than 60 peptide based medicines approved for use by the US Food & Drug Administration, around 140 peptide drugs currently in clinical trials and over 500 in preclinical trials. BOC Sciences emerges as a pioneering force in this domain, offering unparalleled expertise and comprehensive solutions for peptide synthesis. In addition to peptide customization, more synthesis services are also available at BOC Sciences.

What are Peptides?

Peptides are made of short chains of amino acids that are linked by Peptide bonds and are generally consist of 2 up to 50 amino acid residues. They are also important in biological systems as some are signaling molecules, enzyme cofactors, hormones, and antibiotics. The amino acid sequence and in general, the specific arrangement of the AAs among them provide to the peptides their own distinctive biochemical properties, and that allow them to selectively bind biological targets triggering molecular processes in the cell.

Custom Peptide Synthesis Service

BOC Sciences is offering custom-tailored peptide synthesis service, serving all clients in academia, pharmaceutical, and agrobiotech both in the domestic and international markets. Utilizing next-generation facilities and a collection of experienced professionals, BOC Sciences provides custom peptides with unsurpassed levels of quality, purity, and consistency. Our Peptide Synthesis Services includes the following:

Peptide Design

Before any meaningful studies about proteins and peptides can be performed, it is important to consider several factors. The length of sequence, amino acid residues and the sequence of peptides will influence whether correct assembly and purification are feasible. So we need some strategies to design peptide better.

  • Reduce the length of sequence
  • Reduce the number of hydrophobic residues
  • Reduce difficult residues
  • Change the sequence by choosing a different "framework"

Peptide Synthesis

Many synthetic peptides are important commercial or pharmaceutical products ranging from aspartame a dipeptide-sugar substitute to clinically used hormones such as oxytocin adrenocorticotropic hormone and calcitonin. The chemical synthesis of peptides is the condensation of the carboxyl group of one amino acid with the amino group of another amino acid. Protective group strategies are usually necessary to prevent adverse side effects with various amino acid side chains. Thousands of special modifications including: Phosphorylation, Fluorescent dyes, Glycosylation, PEGylation, Rhodamine, Biotin, D-amino acids, stable isotopes, EDANS, Dabsyl, Dansyl, Abz, thiolactic acids, etc.

Solid Phase Peptide Synthesis

The revolution in peptide chemistry of custom peptide synthesis came into being with the invention of solid-phase peptide synthesis (SPPS). In outline, solid-phase peptide synthesis forms peptides by attaching the first alpha-amino acid to a solid resin support.Beefits of solid-phase peptide synthesis are minimal solvent usage, high purity, no racemization for C-terminal residues and automation potential. Introducing a technology characterized by a series of solution-phase synthesis, SPPS differs from the classical one through the stepwise extension of peptide strings with the accomplished immobilized on an immovable resin. Not only does this immobilization approach reduce the need for purification steps but also allows longer and more complicated peptide formation occurs with a higher purity and yield.

The process starts by coupling the first amino acid residue to the resin support which is often a linker with a reactive group like a Chloromethyl or Hydroxymethyl moiety. Additional amino acid residues are added in consecutive steps by coupling reactions, in which after every addition of a single residue, unreacted reagents and by-products are removed by careful washing. In the applicable instances, side- chain protection groups stave off misreactions and uphold amino acid coupling.

Peptide Purification

Although peptide synthesis strategies have been optimized and can be mass produced, the process of peptide synthesis is not perfect. Events such as incomplete deprotection or reactions with free protective groups may result in truncation or deletion of sequences isomers or other by-products. Purification strategies are often based on a combination of separation methods that take advantage of the physicochemical properties of peptides, including size, charge, and hydrophobicity. Purification technologies include:

  • Size exclusion chromatography.
  • Ion exchange chromatography (IEC).
  • Partition chromatography.
  • High performance liquid chromatography (HPLC).

Applications for Synthetic Peptides

Synthetic peptides have a wide variety of applications in drug discovery, vaccine development, and biochemical research.

Drug Discovery and Development

There are hardly any fields as popular as drug discovery and development, where the carved role of synthetic peptides has gained eminence. As a high specificity, low toxicity, and easy modified category of drugs, peptide-based therapeutics are highly attractive. Peptidomimetics are synthetic peptides that can mimic the structure and function of natural peptides, such as agonists, antagonists, enzyme inhibitors, or targeting ligands for disease targets.

Vaccine Development

Synthetic peptides are key components in vaccine design to elicit immune responses against pathogens or tumor cells. Compared with traditional vaccines, peptides have advantages like safety, stability, easy production including that of linear and assembled peptides. The peptides could be designed in such a way that they mimic epitopes of viral, bacterial or cancer antigens and induce both humoral and cellular immune responses. Peptide Vaccines have potential as a solution against such infectious diseases, Autoimmune disease and even cancer.

Molecular Biology and Biomedical Research

The synthetic peptide is a piece of a protein sequence which can be used in research and other applications that require a molecule that is easily altered but still similar to an original protein. Peptides are employed in research as probes, ligands, and inhibitors to study biological processes, discover drug targets, and design clinical assays.

Materials Science and Biomedical Engineering

In addition to their biological applications, synthetic peptides find utility in materials science and biomedical engineering for fabricating biomaterials, drug delivery systems, and tissue engineering scaffolds. Peptide-based materials exhibit biocompatibility, tunable physicochemical properties, and bioactive functionalities.

Why Choose BOC Sciences?

BOC Sciences is specialized in the peptide synthesis services, providing a confidential and efficient service at competitive prices. Every step of peptide synthesis is subject to BOC Sciences' stringent quality control. Typical delivery specifications include:

  • Synthesis of peptides either as crude products or as HPLC-purified material
  • Personalized Ph.D. level consultation with experienced peptide chemistry experts
  • Peptides from 2 to 135 Amino Acids
  • Scales from Nanomole to Milligram to Gram to Kilogram
  • In quantities of several milligrams up to 100 grams
  • Quality assurance: HPLC chromatogram and Mass spec analysis
  • Cyclic Peptides - Cys to Cys, Head to Tail, Internal Lactam
  • Stapled peptides
  • High Throughput Synthesis
  • Additional analyses, such as stability determination, amino acid analysis, and residual solvents etc.
  • Rush Service is Available

Case Study

Exploring the Role of GLP-1 Receptor Agonists in the Brain-Adipocyte Axis

In this case study, researchers investigate the complex neural circuits regulating food intake and energy balance, and how this complexity may hamper efforts to prevent and treat obesity. Key neurons in the hypothalamic nuclei, mainly the arcuate nucleus, control caloric intake and energy expenditure. The populations exhibit orexigenic and anorexigenic neuropeptide expression responding these neurons to neurotrasmitters, hormones, and nutrient signal orchestration. Furthermore, hypothalamic inflammation promoted by fat-rich diets alters the regulation of energy homeostasis inducing resistance to the action of anorexigenic hormones, such as leptin and insulin, and favoring the development of obesity. Recently, glucagon-like peptide-1 receptor agonists (GLP-1RAs), long studied as treatments for type 2 diabetes, are being considered as pharmacologic treatment options for obesity, as evidenced by the more recent approval of liraglutide for weight management. Although GLP-1RAs mainly reduce weight by blunting food intake, there is mounting evidence of additional central effects that merit further clinical investigation.

FAQ

1. What purpose does the peptide bond serve in protein synthesis?

Amino acids are bonded together during protein synthesis by the peptide bond to create a polypetide chain. It's a covalent bond between the carboxyl group of one amino acid, and the amino group of another at the expense of water.

2. What is glucagon-like peptide-1?

Glucagon-like peptide-1 (GLP-1) is a hormone that is released from the small intestine after meals. It can also induce insulin secretion and decrease secretion of glucagon to maintain blood sugar levels. GLP-1 also slows digestion, controls appetite and may have heart protective effects.

3. What are collagen peptides?

Collagen peptides are modified pieces of protein from collagen, which is a main structural protein found in skin, bones, cartilage, connective tissue. When you take collagen in, it is a long chain protein created short chains and amino acids, namely glycine, proline, and hydroxyproline. Hydrolyzed collagen is simply collagen that has been broken down into smaller amount of peptides, or collagen peptides.

Reference

  1. Bruno, G.; et al. Glucagon-Like Peptide-1 Receptor Agonists (GLP-1RAs) in the Brain–Adipocyte Axis. Drugs. 2017, 77: 493–503.
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