Additional Conjugation Services

Protein can be conjugated to fluorescent dyes, drugs, polymers and other proteins, which has a broad range of applications. Protein bioconjugation methods are now extensively used in many chemistry, biology and nanotechnology research groups.

Enzymes, the superior biocatalyst, are involved in a range of biological reactions such as metabolism, DNA replication, gene expression, signal transduction, and so forth. After the conjugation between enzyme and molecular group, it can be applied not only in biotransformation, biosensors, and biomedical engineering fields but also in the production of pharmaceuticals and fine chemicals.

Liposome bioconjugation refers to the conjugation between liposome and oligonucleotides, oligosaccharides, peptides, antibodies or other molecules. After bioconjugation, liposome conjugates can also be used as vehicles to transport nucleic acids, peptides and proteins to cellular site in vivo. They also play an important role in macromolecular carrier, which can encapsulate cosmetic, drugs and fluorescent detection reagents.

Whether nanoparticles or macroparticles can be conjugated with biomolecules such as protein, enzymes, toxins, antibodies, drugs and so on, which have wide application in diagnosis. What’s more, nanoparticle and microparticles can also be applied in agglutination tests and assays, polymerase chain reaction (PCR) tests, particle capture ELISA methods, superparamagnetic-based assays and biosensors.

Site-specific ligation of polyethylene glycol (PEG) can be conjugated with peptide, protein, polymer micelles, antibodies, lectins, enzymes, drugs or nanoparticles, which has numerous promising applications in various fields. To prolong the circulation time of drug, PEGylation can be widely used via the enhanced permeability and retention effect, which provides a new hope for better treatment.

Nano-biological conjugated technology brings hope for the treatment of gliomas and other neurodegenerative diseases. Glioma is the deadliest type of cancer, and the options for treatment are very limited. In December 2010, scientists in the United States and Germany developed a multifunctional Nano-biological conjugated technology, which provided the ability to transport antisense oligodeoxynucleotides (AONs) across the blood-brain barrier (BBB) and the blood-brain tumor barrier (BTB), and targeted their payloads at tumors for receptor-regulated cell uptake, intracellular escape and release into the cytoplasm. Using this technique, the researchers demonstrated an inhibitory effect on the growth of intracranial tumors in a xenograft mouse model of human glioma.

Gold nanoparticles, which are small particles of gold with a diameter of 1 to 100 nm, have high electron density, dielectric properties and catalysis, and can bind to a variety of biological macromolecules without affecting their biological activity. Because spherical gold nanoparticles have strong adsorption function to protein, they can bind to staphylococcal protein A, immunoglobulin, toxin, glycoprotein, enzyme, antibiotic, hormone, bovine serum albumin and so on. Therefore, it has become a very useful tool in basic research and experiments.