Metabolite analysis is essential for understanding biochemical pathways, disease mechanisms, and therapeutic response. BOC Sciences uses state-of-the-art analytical technology and bioinformatics capabilities to provide a full range of metabolite profiling and identification services. With the use of mass spectrometry and nuclear magnetic resonance technologies, BOC Sciences supports biomarker discovery, metabolic pathways, and pharmaceutical research communities with highly precise and actionable data.
Metabolite analysis encounters substantial difficulties because of the vast variety of chemicals and the generally low metabolite concentrations found in biological samples. The detection and quantification of metabolites become unreliable due to these factors which frequently exceed the capability limits of standard analytical methods. Traditional analytical methods often cannot achieve enough sensitivity and specificity to identify metabolites within biological matrices that are complicated by many interfering substances. The existence of isomeric metabolites with identical molecular formulas but different structural arrangements create additional complexity that mandates advanced separation and identification methods. Metabolomics produces extensive high-dimensional data sets that require specialized bioinformatics tools and expert knowledge to interpret correctly. Lack of these resources often causes delays and creates difficulties when trying to extract valuable insights. The current challenges prevent the efficient execution of data-driven decisions in important fields like drug development and medical research which demonstrates the necessity for technological and computational integration.
Our experienced scientific team applies cutting-edge methodologies to ensure high accuracy and reliable metabolite analysis.
We maintain extensive metabolite databases and resources to support multi-level and multidimensional metabolite identification.
Optimized workflows and strategies enhance sample throughput, ensuring timely delivery of analytical results.
We provide flexible, tailored solutions to meet diverse research needs and accelerate project success.
BOC Sciences provides a comprehensive suite of metabolite analysis and identification services designed to support drug metabolism studies, pharmacokinetic profiling, and safety evaluation. Leveraging advanced analytical technologies and deep expertise, we deliver precise and reliable characterization of metabolites across various biological matrices.
We utilize state-of-the-art techniques such as LC-MS/MS, HR-MS, and NMR spectroscopy to accurately elucidate the chemical structures of metabolites, facilitating detailed metabolic pathway analysis.
Our metabolite profiling services include qualitative and quantitative analysis of metabolite spectra in both in vitro and in vivo systems, enabling a comprehensive understanding of compound biotransformation.
BOC Sciences conducts in vitro metabolism studies using liver microsomes, hepatocytes, and recombinant enzymes to identify metabolic products and assess enzyme involvement in drug metabolism.
We perform in vivo metabolite characterization primarily in preclinical animal models to evaluate metabolic pathways, bioavailability, and exposure levels of parent compounds and their metabolites.
Our services include the application of stable isotope-labeled compounds to trace metabolic routes, improve metabolite detection sensitivity, and distinguish between endogenous and drug-derived metabolites.
We focus on identifying and characterizing pharmacologically active metabolites that may contribute to therapeutic efficacy or adverse effects, supporting drug candidate optimization.
BOC Sciences offers specialized screening and identification of potentially toxic or reactive metabolites using trapping agents and advanced analytical platforms to assess safety risks.
Our robust analytical workflows enable sensitive detection and quantification of metabolites in complex biological matrices such as plasma, urine, bile, and tissue homogenates, ensuring comprehensive metabolic profiling.
We provide targeted and untargeted metabolite analysis in various tissue samples, allowing detailed investigation of local metabolism, distribution, and accumulation of drug-related metabolites.
BOC Sciences has developed an integrated platform encompassing in vitro cell systems, in vivo animal models, organoids, and specialized metabolic disease models. These tools are optimized for metabolic stability testing, metabolite profiling, enzyme function analysis, and translational pharmacokinetic research, offering comprehensive support across preclinical drug development.
Model Type | Representative Applications |
Primary Hepatocytes | Human and animal-derived (rat, monkey, mouse); contain full Phase I (CYP450) and Phase II (UGT, SULT) enzymes for metabolic stability and interspecies comparison studies. |
Liver Microsomes | High-throughput metabolic stability screening; suitable for glucuronidation assays (e.g., 7-hydroxycoumarin). |
Caco-2 Cells | Mimic intestinal absorption and first-pass metabolism; used to predict oral drug bioavailability. |
Renal Cells (e.g., HK-2) | Investigate renal metabolic pathways and excretion mechanisms. |
Brain Endothelial Cells (e.g., hCMEC/D3) | Evaluate blood-brain barrier permeability and CNS drug metabolism. |
Model Type | Representative Applications |
Liver Organoids | Long-term culture models maintaining primary hepatocyte function; used for metabolic phenotype analysis. |
Tumor Organoids | Cancer-specific models for studying drug metabolism, resistance, and metabolic reprogramming in tumor microenvironments. |
Species/Model | Applications |
Rodents (Rat, Mouse) | Routine PK studies with plasma, urine, and bile collection; support for full metabolite spectrum analysis. |
Knockout Mouse Models | Gene-edited strains (e.g., CYP450 knockout) used for validating enzyme-specific metabolism. |
Non-Human Primates (Macaques) | High homology to human metabolic enzymes; ideal for translational research in metabolic diseases (e.g., obesity, diabetes). |
Pregnancy Models in NHPs | Metabolomics of placenta, fetal liver, and adaptive tissue metabolism during gestation. |
BOC Sciences integrates state-of-the-art analytical technologies to provide accurate, efficient, and comprehensive metabolite analysis and identification services. By combining multiple orthogonal techniques such as high-resolution mass spectrometry, ion mobility, gas chromatography, and nuclear magnetic resonance, we ensure structural elucidation of known and unknown metabolites across biological matrices. Our platform supports both qualitative and quantitative analysis in various applications, including in vitro/in vivo metabolism, toxicity evaluation, and stable isotope tracing studies.
BOC Sciences offers a robust analytical package designed to decode the complexity of metabolite identity, kinetics, and biological relevance. Our deliverables combine high-resolution instrumentation with curated databases and bioinformatics support.
Engage with clients to clarify research goals, sample types, and expected data outputs (e.g., metabolite profiling, structure identification, biomarker discovery). Define project scope and determine if the study requires targeted or untargeted metabolomics.
Design a tailored analytical plan based on the research objective. Select appropriate platforms (e.g., LC-MS, GC-MS), ionization modes, and sample preparation methods to ensure optimal metabolite coverage and data quality.
Process biological samples using validated protocols for metabolite extraction and derivatization (if applicable). Acquire high-resolution data through established instrumentation (e.g., Q-TOF, NMR) under controlled experimental settings.
Apply advanced algorithms and software tools for peak alignment, deconvolution, and metabolite identification. Utilize MS/MS fragmentation patterns, isotopic ratios, and spectral libraries to support accurate structural assignments.
Conduct statistical and pathway-level analysis to reveal biologically relevant changes. Identify differentially expressed metabolites and link them to metabolic pathways, enzymatic reactions, or potential disease mechanisms.
Deliver a comprehensive data package that includes raw data files, processed result tables, annotated spectra, concentration-time curves, and detailed interpretation summaries. Reports are tailored to support scientific publication and inform subsequent experimental design decisions.
Metabolite analysis refers to the comprehensive identification and quantification of small molecule metabolites within biological samples. It provides critical insights into cellular processes, disease mechanisms, and biochemical pathways, supporting applications such as biomarker discovery, drug development, and systems biology research.
Metabolites are typically identified using advanced analytical techniques such as mass spectrometry (MS) coupled with chromatography (GC-MS or LC-MS) and nuclear magnetic resonance (NMR) spectroscopy. These methods enable high-resolution detection, structural elucidation, and quantification of metabolites by comparing sample data against reference databases and spectral libraries.
Metabolite analysis is crucial in biomedical research because it reflects real-time physiological and pathological states. By profiling metabolic changes, researchers can uncover disease biomarkers, understand drug effects, and tailor personalized treatments. This approach enhances the understanding of complex biological systems and accelerates translational medicine.
Common challenges in metabolite analysis include the chemical diversity and dynamic range of metabolites, sample complexity, and data interpretation. Overcoming these requires optimized sample preparation, advanced instrumentation, robust data processing algorithms, and comprehensive metabolite databases to ensure accurate and reproducible results.